You are watching: Why is heat fixation important in the staining procedure?
If a straightforward stain of only one kind of bacteria, or solitary mixed sample of bacteria is being prepared, no separation is required.
Article Summary: In bespeak to see individual bacteria with a light microscope, a bacterial smear have to be attached come a slide and then stained. Below is the procedure.
In bespeak to warmth fix a bacter smear, it is vital to an initial let the bacter sample wait dry. Then either place the slide in the on slide holder that a microincinerator, or happen the dried slide through the fire of a Bunsen burner 3 or 4 times, smear side encountering up. As soon as the on slide is warmth fixed, it have the right to then it is in stained.
In bespeak to be able to clearly see separation, personal, instance bacteria, a sample the a bacterial colony must be mixed into water or physiological saline. This helps to evenly spread out out the bacterial sample.
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Bacterial smear after warmth fixing and before staining. Positive bacterial control in one on the left, an adverse control top top right, and unknown bacteria in center circle.
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